王静云

个人信息Personal Information

教授

博士生导师

硕士生导师

性别:女

毕业院校:大连理工大学

学位:博士

所在单位:生物工程学院

学科:生物化工. 生物工程与技术. 药物工程

办公地点:生物楼512

联系方式:wangjingyun67@dlut.edu.cn

电子邮箱:wangjingyun67@dlut.edu.cn

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Lipase Immobilization onto the Surface of PGMA-b-PDMAEMA-grafted Magnetic Nanoparticles Prepared via Atom Transfer Radical Polymerization

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论文类型:期刊论文

发表时间:2014-11-01

发表刊物:CHINESE JOURNAL OF CHEMICAL ENGINEERING

收录刊物:SCIE、EI、ISTIC、Scopus

卷号:22

期号:11-12

页面范围:1333-1339

ISSN号:1004-9541

关键字:Enzyme; Atom transfer radical polymerization; Immobilized lipase; Fe3O4 nanoparticles

摘要:A block copolymer of 2-dimethylaminoethyl methacrylate (DMAEMA) and glycidyl methacrylate (GMA) was grafted onto the surface of magnetic nanoparticles (Fe3O4) via atom transfer radical polymerization. The resultant PGMA-b-PDMAEMA-grafted-Fe3O4 magnetic nanoparticles with amino and epoxy groups were characterized by Fourier transform infrared spectroscopy, powder X-ray diffraction, thermo-gravimetric analysis, and scanning electron microscopy. Lipase from Burkholderia cepacia was successfully immobilized onto the magnetic nanoparticles by physical adsorption and covalent bonding. The immobilization capacity of the magnetic particles is 0.5 mg lipase per mg support, with an activity recovery of up to 43.1% under the optimum immobilization condition. Biochemical characterization shows that the immobilized lipase exhibits improved thermal stability, good tolerance to organic solvents with high lg P, and higher pH stability than the free lipase at pH 9.0. After six consecutive cycles, the residual activity of the immobilized lipase is still over 55% of its initial activity. (C) 2014 The Chemical Industry and Engineering Society of China, and Chemical Industry Press. All rights reserved.