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论文类型：期刊论文

发表时间：2014-04-14

发表刊物：CHEMISTRY-A EUROPEAN JOURNAL

收录刊物：SCIE、EI、PubMed、Scopus

卷号：20

期号：16

页面范围：4691-4696

ISSN号：0947-6539

关键字：dyes; fluorescent probes; imaging agents; steric hindrance; viscosity

摘要：Cellular viscosity is a critical factor in governing diffusion-mediated cellular processes and is linked to a number of diseases and pathologies. Fluorescent molecular rotors (FMRs) have recently been developed to determine viscosity in solutions or biological fluid. Herein, we report a distorted-BODIPY-based probe BV-1 for cellular viscosity, which is different from the conventional pure rotors. In BV-1, the internal steric hindrance between the meso-CHO group and the 1,7-dimethyl group forced the boron-dipyrrin framework to be distorted, which mainly caused nonradiative deactivation in low-viscosity environment. BV-1 gave high sensitivity (x=0.62) together with stringent selectivity to viscosity, thus enabling viscosity mapping in live cells. Significantly, the increase of cytoplasmic viscosity during apoptosis was observed by BV-1 in real time.