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论文类型：期刊论文

发表时间：2015-07-07

发表刊物：ANALYST

收录刊物：SCIE、PubMed、Scopus

卷号：140

期号：13

页面范围：4594-4598

ISSN号：0003-2654

摘要：Hypochlorous acid (HClO)/hypochlorite (ClO-), one of the reactive oxygen species (ROS), is a key microbicidal agent used for natural defense; however, HClO is also responsible for some human diseases. Although much effort has been made to develop HClO-selective fluorescent probes, many of them display a delayed response time and nanomole-sensitive probes are rare. In this study, we designed and synthesized an aza-coumarin based fluorescent probe AC-ClO for ClO- determination with fast response (completed within 2 min) and high sensitivity (detection limit is 25 nM). AC-ClO displayed a color change from pink to light yellow and a remarkable "turn-on" fluorescence response towards ClO-. Confocal fluorescence microscopy experiments demonstrated that the probe could be applied for the live-cell imaging of exogenous and endogenous ClO-.