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论文类型：期刊论文

发表时间：2015-02-16

发表刊物：ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

收录刊物：SCIE、EI、PubMed、Scopus

卷号：54

期号：8

页面范围：2510-2514

ISSN号：1433-7851

关键字：cancer sensing; dyes; mitochondria; polarity; ratiometric imaging

摘要：Mitochondrial polarity strongly influences the intracellular transportation of proteins and interactions between biomacromolecules. The first fluorescent probe capable of the ratiometric imaging of mitochondrial polarity is reported. The probe, termed BOB, has two absorption maxima ((abs)=426 and 561nm) and two emission maximaa strong green emission ((em)=467nm) and a weak red emission (642nm in methanol)when excited at 405nm. However, only the green emission is markedly sensitive to polarity changes, thus providing a ratiometric fluorescence response with a good linear relationship in both extensive and narrow ranges of solution polarity. BOB possesses high specificity to mitochondria (R-r=0.96) that is independent of the mitochondrial membrane potential. The mitochondrial polarity in cancer cells was found to be lower than that of normal cells by ratiometric fluorescence imaging with BOB. The difference in mitochondrial polarity might be used to distinguish cancer cells from normal cells.