王静云

个人信息Personal Information

教授

博士生导师

硕士生导师

性别:女

毕业院校:大连理工大学

学位:博士

所在单位:生物工程学院

学科:生物化工. 生物工程与技术. 药物工程

办公地点:生物楼512

联系方式:wangjingyun67@dlut.edu.cn

电子邮箱:wangjingyun67@dlut.edu.cn

扫描关注

论文成果

当前位置: 中文主页 >> 科学研究 >> 论文成果

Esterification degree of fructose laurate exerted by Candida antarctica lipase B in organic solvents

点击次数:

论文类型:期刊论文

发表时间:2015-02-01

发表刊物:ENZYME AND MICROBIAL TECHNOLOGY

收录刊物:SCIE、EI、PubMed、Scopus

卷号:69

页面范围:46-53

ISSN号:0141-0229

关键字:Sugar ester of fatty acid; Degree of esterification; Candida antarctica lipase B; Fructose laurate; ATR-FTIR

摘要:Sugar esters of fatty acids have many applications as biocompatible and biodegradable emulsifiers, which are determined by their degrees of esterification (DE). Direct esterification of fructose with lauric acid in organic media used commercial immobilized Candida antarctica lipase B (CALB) was investigated for DE. Significant difference of DE was observed between 2-methyl-2-butanol (2M2B) and methyl ethyl ketone (MEK), as di-ester/mono-ester molar ratio of 1.05:1 in 2M2B and 2.79:1 in MEK. Fourier transform infrared (FTIR) spectra showed that the secondary structure of the enzyme binding mono-ester presented distinct difference in 2M2B and MEK. Contents of beta-turn and antiparallel beta-sheet of CALB in 2M2B were 26.9% and 16.2%, respectively, but 19.1% and 13.2% in MEK. To understand the relationship between the conformational changes and differences of DE, mono-ester and fatty acid were directly employed for synthesis of di-ester. The maximum initial velocity of di-ester synthesis in MEK was 0.59 mmol g(enzyme)(-1) h(-1), which was 2.19-fold as greater as that in 2M2B, indicating that CALB conformation in MEK was preferred for the synthesis of di-ester. These results demonstrated that the conformation of CALB binding mono-ester affected by organic solvents essentially determined DE. (C) 2014 Elsevier Inc. All rights reserved.