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个人信息Personal Information
教授
博士生导师
硕士生导师
性别：女
毕业院校：大连理工大学
学位：博士
所在单位：生物工程学院
学科：生物化工. 生物工程与技术. 药物工程
办公地点：生物楼512
联系方式：wangjingyun67@dlut.edu.cn
电子邮箱：wangjingyun67@dlut.edu.cn
Efficient enhancement of DNA cleavage activity by introducing guanidinium groups into diiron(III) complex
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论文类型：期刊论文
发表时间：2008-01-01
发表刊物：BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
收录刊物：SCIE、PubMed
卷号：18
期号：1
页面范围：109-113
ISSN号：0960-894X
摘要：Inspired by the structures of natural nucleases, guanidinium groups were introduced into binuclear iron(III) systems. Compared with the corresponding analogue without guanidinium groups, the new diiron(III) system led to considerable rate enhancement on DNA cleavage. The cooperativity between metal ions and guanidine groups was evidenced by the fact that no significant cleavage was observed after incubating pBR322 plasmid DNA with non-metalated ligands or free Fe3+ ion. DNA binding experiments indicated that introduction of positively charged guanidinium, groups can obtain more than one order of magnitude enhancement in the affinity of complex with DNA. (C) 2007 Elsevier Ltd. All rights reserved.